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MME Antikörper (AA 501-600)

Name: MME Antikörper (AA 501-600)
SKU: ABIN669666
Price: 357.7 EUR
Availability: InStock

Dieser Anti-MME Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von MME in WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (p) und IF (cc). Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-CD10 Polyclonal Antibody (ABIN669666), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (MME Antikörper (AA 501-600))

IHC

Human Hela cells probed with CD10 Polyclonal Antibody, Alexa Fluor 647 conjugated -A647) (green) at 1:20 for 30 minutes compared to unstained cells (blue) and isotype control (orange). (MME Antikörper (AA 501-600))

FACS

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD10 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (MME Antikörper (AA 501-600))

IHC (p)

A549 cells probed with CD10 Polyclonal Antibody, Unconjugated at 1:20 for 30 minutes followed by incubation with a conjugated secondary -PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (MME Antikörper (AA 501-600))

FACS

5 Abbildungen

(1 reference)

Produktnummer ABIN669666

Kurzübersicht für MME Antikörper (AA 501-600) (ABIN669666)

Target

Alle MME Antikörper anzeigen

MME (Membrane Metallo-Endopeptidase (MME))

Reaktivität

Human, Ratte, Maus

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser MME Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Discover our top product MME Primärantikörper

Bindungsspezifität
36

12

10

8

8

8

6

6

5

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 501-600

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CD10

Isotyp

IgG
anti-Membrane Metallo-Endopeptidase (MME) (AA 52-352) antibody
MME Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-246) antibody
MME Reaktivität: Human WB, IHC, ELISA Wirt: Maus Monoclonal 7D4B1 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 382-538) antibody
MME Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reaktivität: Human WB, IHC, StM Wirt: Maus Monoclonal MME-1870 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 286-428) antibody
MME Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Membrane Metallo-Endopeptidase (MME) antibody (FITC)
MME Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-78 FITC

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME Reaktivität: Human FACS, IHC, ELISA Wirt: Maus Monoclonal 3G6B11 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibody
MME Reaktivität: Human WB, FACS, ELISA Wirt: Maus Monoclonal 6G12E12 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibody
MME Reaktivität: Human WB, IHC, ELISA, StM, Coat Wirt: Maus Monoclonal MME-1892 unconjugated

anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
MME Reaktivität: Human, Ratte WB, FACS, IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Chopra, Kraus, Schwinn, Ritz, Mattenheimer, Mottok, Rosenwald, Einsele, Beilhack: "Non-invasive bioluminescence imaging to monitor the immunological control of a plasmablastic lymphoma-like B cell neoplasia after hematopoietic cell transplantation." in: PLoS ONE, Vol. 8, Issue 12, pp. e81320, (2013) (PubMed).
Target

MME (Membrane Metallo-Endopeptidase (MME))

Andere Bezeichnung

CD10

Substanzklasse

Chemical

Hintergrund

Synonyms: NEP, SFE, CD1, CALLA, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Neutral endopeptidase, Skin fibroblast elastase, MME, EPN
Background: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 3 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Gen-ID

4311

UniProt

P08473

Pathways

RTK Signalweg, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration